MiR-5195-3p targets the PCBP2/PI3K/AKT pathway to inhibit melanoma cell proliferation and migration

Although miR-5195-3p has been acknowledged for its tumor suppressor role in diverse cancer categories, precise functions and mechanisms concerning melanoma have not comprehensively elucidated. In this study, we employed quantitative reverse transcription PCR, Western blot analysis, immunohistochemistry staining to investigate the expression patterns of poly (rC) binding protein 2 (PCBP2) tissues compared adjacent tissues. Our findings revealed downregulation upregulation PCBP2 Through implementation a luciferase reporter assay, successfully identified as newly discovered target cells. Enforced via mimics inhibited cell proliferation migration A375 A2058 cells, demonstrated by CCK-8 transwell assays. reintroduction partially reversed inhibitory effects overexpression. Treatment with LY294002, an inhibitor PI3K/AKT signaling pathway, also Furthermore, our results suggest that inhibits activation pathway inhibiting PCBP2. conclusion, research targeting PCBP2-mediated potential therapeutic treatment.